Cellular stress in vitro and longevity in vivo
Dekker, P.
Citation
Dekker, P. (2012, February 28). Cellular stress in vitro and longevity in vivo. Retrieved from https://hdl.handle.net/1887/18532
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
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Cellular stress in vitro and longevity in vivo
Pim Dekker
Financial support for the publication of this thesis by the Nederlandse Vereniging voor Gerontologie (Dutch Society for Gerontology) and by Unilever PLC is gratefully acknowledged.
© Pim Dekker, 2011
No part of this thesis may be reproduced, stored in a retrieval system or transmitted in any form or by any means, without permission of the author or, when appropriate, of the publisher of publications.
ISBN: 978-94-6182-063-1
This research was funded by the Netherlands Genomics Initiative (NCHA 050-060-810), the Innovation Oriented research Program on Genomics (SenterNovem; IGE01014 and IGE5007), the Netherlands Genomics Initiative/Netherlands Organization for scientific research (NGI/NWO;
05040202 and 050-060-810), EU funded Network of Excellence Lifespan (FP6 036894) and Unilever PLC.
Cover design and layout: Gijs Grob
With courtesy of AMPELMANN GmbH. The design company specializes in emotional lifestyle products with high utility value. More information under: www.ampelmann.de
Printed by: Off Page, Amsterdam
Cellular stress in vitro and longevity in vivo
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof. mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op dinsdag 28 februari 2012 klokke 16.15 uur
door
Pim Dekker geboren te Rotterdam
in 1973
Promotie commissie
Promotores: Prof. Dr. R.G.J. Westendorp Prof. Dr. H.J. Tanke
Co-promotores: Dr. A.B. Maier Dr. D. van Heemst
Referenten: Prof. Dr. P.D. Adams (Glasgow University, UK) Prof. Dr. P.E. Slagboom
Prof. Dr. A.M. Deelder
A very popular error:
having the courage of one's convictions;
rather it is a matter of having the courage for an attack on one's convictions
F. Nietzsche
Contents
Chapter 1. General introduction 9
Chapter 2. Rapid flow cytometric method for measuring 19 Senescence Associated-β-galactosidase activity in human
fibroblasts
Chapter 3. Stress-induced responses of human skin fibroblasts in 39 vitro reflect human longevity
Chapter 4. Relation between maximum replicative capacity and 61 oxidative stress-induced responses in human skin fibroblasts
in vitro
Chapter 5. Chronic inhibition of the respiratory chain in human fibroblast 79 cultures: Differential responses related to subject chronological
and biological age
Chapter 6. Microarray-based identification of age-dependent differences 103 in gene expression of human dermal fibroblasts
Chapter 7. Human in vivo longevity is reflected in vitro by differential 137 metabolism as measured by 1H-NMR profiling of cell culture
supernatants
Chapter 8. General discussion 165
Nederlandse samenvatting 175
List of publications 179
Dankwoord 180
Curriculum Vitae 181
