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Cover Page The following handle holds various files of this Leiden University dissertation: http://hdl.handle.net/1887/81582

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The following handle holds various files of this Leiden University dissertation: http://hdl.handle.net/1887/81582

Author: Horton, A.A.

Title: Towards a greater understanding of the presence, fate and ecological effects of microplastics in the freshwater environment

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Towards a greater understanding of the

presence, fate and ecological effects of

microplastics in the freshwater environment

Alice A. Horton

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Towards a greater understanding of the presence, fate and ecological effects of

microplastics in the freshwater environment

Proefschrift ter verkrijging van

de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,

volgens besluit van het College van Promoties te verdedigen op donderdag 19 december 2019

klokke 13.45 uur door

Alice Avory Horton geboren te Romsey, UK

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Promotiecommisie:

Promotor: Prof. dr M. G. Vijver (Leiden University, the Netherlands)

Co-promotors: Prof. dr. P. M. van Bodegom (Leiden University, the Netherlands) Dr. E. Lahive (Centre for Ecology and Hydrology, UK)

Overige leden: Prof. dr. A. Tukker (Leiden University, the Netherlands)

Prof dr. W. J. G. M. Peijnenburg (Leiden University, the Netherlands)

Prof dr. A. A. Koelmans (Wageningen University and Research, the Netherlands) Dr. H. Feuchtmayr (Centre for Ecology and Hydrology, UK)

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Contents

SUMMARY ... 1

SAMENVATTING ... 4

CHAPTER 1 - Introduction ... 7

CHAPTER 2 - Microplastics in freshwater and terrestrial environments: evaluating the current understanding to identify the knowledge gaps and future research priorities ... 29

CHAPTER 3 - Large microplastic particles in sediments of tributaries of the River Thames, UK – abundance, sources and methods for effective quantification ... 78

CHAPTER 4 - The influence of exposure and physiology on microplastic ingestion by the freshwater fish Rutilus rutilus (roach) in the River Thames, UK ... 106

CHAPTER 5 - Acute toxicity of organic pesticides to Daphnia magna is unchanged by co-exposure to polystyrene microplastics ... 133

CHAPTER 6 - Accumulation of polybrominated diphenyl ethers and microbiome response in the great pond snail Lymnaea stagnalis with exposure to nylon (polyamide) microplastics.. ... 171

CHAPTER 7 - Discussion ... 208

ACKNOWLEDGEMENTS ... 235

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