Functional genomics to study protein secretion stress in Aspergillus niger
Silva Pinheiro Carvalho, N.D.
Citation
Silva Pinheiro Carvalho, N. D. (2011, June 7). Functional genomics to study protein secretion stress in Aspergillus niger. Retrieved from https://hdl.handle.net/1887/17685
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/17685
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Functional genomics to study protein secretion
stress in Aspergillus niger
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Functional genomics to study protein secretion stress in Aspergillus niger
Proefschrift
ter verkrijging van de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof. mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op dinsdag 7 Juni 2011 klokke 11:15 uur
door
Neuza Daniela Silva Pinheiro Carvalho geboren te Fafe, Portugal
in 1983
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Promotiecommissie
Promotor: Prof. Dr. C.A.M.J.J. van den Hondel Co-promotor: Dr. A.F.J. Ram
Overige leden: Prof. Dr. H.P. Spaink (Leiden University) Prof. Dr. P.J. Punt (TNO/Leiden University) Prof. Dr. V. Meyer (Technical University Berlin) Prof. Dr. H. Wösten (University of Utrecht) Prof. Dr. D. Archer (University of Nottingham)
The work described in this thesis was financially supported by the Kluyver Centre for Genomics of Industrial Fermentations and carried out at the Institute of Biology in the department Molecular Microbiology and Biotechnology at Leiden University.
Cover: From left to right: A. niger colony; bioreactor for fungi cultivation; ER-GFP tagged Layout: Neuza Carvalho
Printed by: Wöhrmann Print Service, Zutphen, The Netherlands ISBN: 978-90-8570-751-6
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Aos meus queridos pais:
Domingos e Adelina
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Contents
Chapter 1
General introduction 9
Aim and thesis outline 25
Chapter 2
Expanding the ku70 toolbox for filamentous fungi: establishment of
complementation vectors and recipient strains for advanced gene analyses 27 Chapter 3
Functional YFP-tagging of the essential GDP-mannose transporter reveals an important role for the secretion related small GTPase SrgC protein in
maintenance of Golgi bodies in Aspergillus niger 45 Chapter 4
Effects of a defective ERAD pathway on growth and heterologous protein
production in Aspergillus niger 63
Chapter 5
Genome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger reveals a coordinated cellular
response to counteract ER stress 87
Chapter 6
General discussion 111
Summary 123
Samenvatting 125
Supplementary material 127
References 129
Publications 153
Curriculum Vitae 154
Acknowledgments 155
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