Post-incision events induced by UV : regulation of incision and the role of post-incision factors in mammalian NER
Overmeer, R.M.
Citation
Overmeer, R. M. (2010, September 29). Post-incision events induced by UV : regulation of incision and the role of post-incision factors in mammalian NER. Retrieved from
https://hdl.handle.net/1887/15997
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
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Stellingen
Behorend bij het proefschrift:
Post-incision events induced by UV
Regulation of incision and the role of post-incision factors in mammalian NER
1. RPA tightly regulates NER mediated incisions thereby averting generation of DNA strand breaks that could lead to mutagenic and recombinogenic events. (This thesis) 2. Recruitment of DNA polymerase δ to the NER incision complex is dependent on
RFC1 even in the presence of the sliding clamp PCNA. (This thesis)
3. Incision at sites of 6-4 photoproducts by Ape1 leads to unrepairable nicks. (This thesis)
4. The dynamic association/dissociation of GFP-RFCp140 with stalled NER complexes suggests that the residence time at sites of damage does not necessarily refl ect the time to fulfi l their function but rather a measure for their affi nity to such complexes.
(This thesis)
5. Effi cient nucleotide excision repair synthesis requires a surprisingly high number of different DNA polymerases. (This thesis)
6. Phosphorylation of RPA is a likely mediator of ATR dependent NER during S phase.
(Auclair Y et al. (2008), Proc. Natl. Acad. Sci. U. S A 105, 17896-17901; Patrick SM et al. (2005), Biochemistry, 44, 8738-8448)
7. ELG1 regulates the deubiquitinatination of PCNA in mammalian cells. (Lee KY et al., J Biol Chem. 2010 Apr 2;285(14):10362-9)
8. It is surprising that the accumulation of CPDs on telomeres does not enhance telomere shortening. (Rochette PJ, Brash, DE, PLoS Genet. 2010 Apr 29;6(4))
9. The SUMO pathway is essential for effi cient homologous recombination. (Ouyang KJ et al., PLoS Biol. 2009 Dec;7(12); Sacher M et al., Nat Cell Biol. 2006 Nov;8(11):1284-90)
10. The high level of competition and the strong emphasis on high ranking publications could potentially impair open scientifi c communication.
11. The next step in female emancipation should be sought in granting equal rights and obligations to males as opposed to positive discrimination of females.
12. “Employ your time in improving yourself by other men’s writings so that you shall come easily by what others have labored hard for” (Socrates)
13. “It is not enough to have a good mind; the main thing is to use it well.” (René Descartes)
René M. Overmeer 2010
