University of Groningen
Single-molecule enzymology with a ClyA nanopore
Galenkamp, Nicole
DOI:
10.33612/diss.130258760
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Galenkamp, N. (2020). Single-molecule enzymology with a ClyA nanopore. University of Groningen. https://doi.org/10.33612/diss.130258760
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Propositions
Belonging to the thesisSingle-molecule enzymology with a ClyA nanopore
Nicole Stéphanie Galenkamp
1. The nanopore technique is a sensitive method to observe at the single-molecule level biomolecules and has the power to resolve fine details about them. (This thesis)
2. Enzymes have complex and inhomogeneous dynamics that can be characterized and analyzed with single-molecule research. (Chapter 1)
3. The single-molecule nanopore technique is not only useful for fundamental research, but could be a powerful tool in developing next-generation sensors for metabolomics analysis. (Chapter 2)
4. Substrate binding en turnover in enzymes can lower the energy barriers for conformational exchange in enzymes. (Chapter 3 and 4)
5. Understanding the principles of enzyme dynamics can be exploited for re-engineering known enzymes or even for developing artificial enzymes. (Chapter 3 and 4)
6. Introduction of a single mutation to the glucose binding protein influences the affinity. (Chapter 5)
7. Solvent organization is a key contributor to the thermodynamics of protein-ligand binding. (Chapter 5)
8. “Be an enzyme – a catalyst for change. As a slogan, I don’t know if that’s ever going to be right up there with Ich Bin Ein Berliner, or “I have a dream,” but there’s a lot of truth to it” – Pierre Omidyar
