Costimulation blockade and regulatory T-cells in a non- human primate model of kidney allograft transplantation Haanstra, K.G.

Citation

Haanstra, K. G. (2008, March 13). Costimulation blockade and regulatory T- cells in a non-human primate model of kidney allograft transplantation.

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Costimulation blockade and regulatory T-cells in a non-human primate model of kidney allograft

transplantation

Krista Haanstra

biology of the Biomedical Primate Research Centre, Rijswijk, The Netherlands, and was in part financed by PanGenetics

Financial support for the publication of this thesis was provided by the Dutch Kidney Foundation (Nierstichting), the Dutch Transplantation Society (NTV), Pan- Genetics and the BPRC.

Cover Henk van Westbroek Lay-out Jeffry Ammeraal

Printed by Gildeprint Drukkerijen BV, Enschede ISBN Number 978-90-9022639-2

Costimulation blockade and regulatory T-cells in a non-human primate model of kidney allograft

transplantation

Proefschrift

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. P.F. van der Heijden,

volgens besluit van het College voor Promoties te verdedigen op donderdag 13 maart 2008

klokke 13:45 uur

door

Krista Geraldine Haanstra

geboren te Zwolle in 1975

Prof. dr. R.J.M. ten Berge

Amsterdam Medical Centre, Amsterdam Prof. dr. J.W. de Fijter

Contents

1 Introduction 5

2 Prevention of kidney allograft rejection using anti-CD40 and anti-

CD86 in primates 47

3 Costimulation blockade followed by a 12-week period of Cyclosporine A facilitates prolonged drug-free survival of rhesus monkey kidney

allografts 65

4 No synergy between ATG induction and costimulation blockade in-

duced kidney allograft survival in rhesus monkeys 75 5 Expression patterns of regulatory T-cell markers in accepted and re-

jected non-human primate kidney allografts 93

6 Characterisation of naturally occurring CD4⁺CD25⁺regulatory T-cells

in rhesus monkeys 119

7 Summary and discussion 137

Abbreviations 161

Nederlandse samenvatting 163

Curriculum vitae 167

Dankwoord 169

List of publications 171

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