Cover Page
The handle http://hdl.handle.net/1887/20749 holds various files of this Leiden University dissertation.
Author: Hashemi Shabestari, Maryam
Title: Spin-label EPR on disordered and amyloid proteins
Issue Date: 2013-04-16
Publications
133
PUBLICATIONS
Journal papers
2012 Scanu S., Förster J., Finiguerra M., Hashemi Shabestari M., Huber M., Ubbink M. “The complex of cytochrome f and plastocyanin from Nostoc sp. PCC 7119 is highly dynamic”.
Chembiochem. (2012), 13: 1312-8.
2011 Carlotto S., Zerbetto m., Hashemi Shabestari M., Toniolo C., Huber M., Polimeno A. “In Silico interpretation of cw- ESR at 9 and 95 GHz of Mono- and bis- TOAC labeled Aib- homopeptides in fluid and frozen acetonitrile”. J. Phys.Chem.
B. (2011), 115: 13026-36.
2006 Hashemi Shabestari M., Sarbolouki M.N. “Novel transdermal drug delivery systems”. Iranian Journal of Dermatology. (2006), 9: 270-283.
Published abstracts
2013 Hashemi Shabestari M., Wolfs C., Spruijt R., van
Amerongen H., Huber M. “The disordered N-terminus of the plant antenna protein CP29 studied by EPR- is this 100 residue stretch unstructured”. Biophysical Journal. (2013), 104: p219a.
2013 Hashemi Shabestari M., Segers-Nolten I.M.J., Meeuwenoord N.J., Filippov D.V., Claessens M.M.A.E., van Rooijen B.D., Subramaniam V., Huber M. “Aggregation and membrane interaction of alpha-synuclein and amyloid β by electron
paramagnetic resonance”. Biophysical Journal. (2013), 104: p52a.
2012 Hashemi Shabestari M., Segers-Nolten I.M.J., Subramaniam V., Huber M. “Elucidating the αSynuclein Fibril Fold by Pulsed EPR”.
Biophysical Journal. (2012), 102: 441a.
2012 Hashemi Shabestari M., Meeuwenoord N.J., Filippov D.V., Huber M. “The effect of a membrane mimicking detergent on Alzheimer’s amyloid peptide aggregation studied by EPR”.
Biophysical Journal. (2012), 102: 454a.
2010 Hashemi Shabestari M., Sepkhanova I., Drescher M.,
Meeuwenoord N.J., Filippov D.V., Limpens R., Koning R.I.,
Huber M. “Detecting the aggregation of amyloid peptides by
Spin Label EPR”. Biophysical Journal. (2010), 98: 458a.
Publications
In preparation
2013 Hashemi Shabestari M., van Son M., Moretto A., Crisma M., Toniolo C., Huber M. “Structure and first EPR characterization of helical peptides with TOAC spin-labels: models for short
distances”.
2013 Hashemi Shabestari M., Meeuwenoord N.J., Filippov D.V., Huber M. “The interaction of amyloid β peptide with a membrane mimicking detergent using EPR”.
2013 Hashemi Shabestari M., Plug T., Motazacker M.M.,
Meeuwenoord N.J., Filippov D.V., Meijers J., Huber M. “The
aggregation potential of the 1-15 and 1-16 fragments of the
amyloid β peptide and their influence on the aggregation of
Aβ40”.
Curriculum Vitae
135
CURRICULUM VITAE
Maryam Hashemi Shabestari was born on September 21, 1979 in Tehran, Iran.
After graduating from high school in 1997, she studied plant biology at the Faculty of Sciences at Tehran University, and received her bachelor’s degree (with honor) in 2001. She obtained her MSc. degree in biophysics in 2004 from the Institute of Biophysics and Biochemistry at Tehran University with a focus on preparation and evaluation of lipid formulations in transdermal drug delivery. Her MSc. thesis work was presented at the “European conference on drug delivery and pharmaceutical technology” in Seville, Spain in 2004 and at the “49
thannual meeting of Biophysical Society” in Long Beach, California in 2005. She then worked as a research assistant at the Osvah (ex IRAN-Merck) pharmaceutical company and as a part-time instructor at Tehran University.
In 2008, she started her PhD at the Department of Molecular Physics at Leiden University under the supervision of Prof. dr. Edgar Groenen and Dr. Martina Huber.
Her research concerned determining the structure, dynamics, and interaction of proteins, specifically fibril-forming proteins involved in neurodegenerative disorders, using advanced Electron Paramagnetic Resonance (EPR) methods.
During her PhD period, she contributed to the teaching at the Department of
Physics by assisting the 2
ndyear and 3
rdyear physics course for biology students
and supervised a four month bachelor project in the Molecular Physics group. She
was also involved in the “LAPP-Top program (Leiden Advanced Pre-university
Program for Top students). In addition, she has attended several national and
international workshops and training courses. She has presented the results of her
research orally at the NWO study group meeting on Protein research, Nucleic acids,
Lipids, and Biomembranes (2010, Veldhoven, Netherlands), at the 44
thAnnual
International Meeting of the ESR Spectroscopy Group of the Royal Society of
Chemistry (2011, York, UK), at the 19
thmeeting of the Benelux EPR Society
(2011, Antwerp, Belgium), and at the physics@FOM meeting (2013, Veldhoven,
Netherlands). Besides several national symposia and meetings, her work was
presented as a poster at the 7
thEuropean Federation of EPR (2009, Antwerp,
Belgium), and at the 5
thEF-EPR Summer School on Advanced EPR Spectroscopy
(2010, Konstanz, Germany). In November 2010, she was awarded the 2010 Holland
Research School of Molecular Chemistry (HRSMC) poster prize with a project
titled: “Aggregation of transmembrane peptides studied by spin-label EPR”. Her
PhD program was supported by the “Stichting voor Fundamenteel Onderzoek der
Materie (FOM)” and resulted in this thesis.
Curriculum Vitae
Acknowledgement
137
ACKNOWLEDGEMENT
Although the cover of this thesis bears only my name, many people have contributed to the work in this thesis. I gladly acknowledge their contribution here.
First of all, I would like to thank Dr. Martina Huber for giving me the opportunity to do a PhD in the MoNOS group. There is no PhD trajectory without ups and downs, and I greatly appreciate the strong support and wise decisions of Prof. Edgar Groenen during my PhD period, which helped me to stay on track.
I would like to acknowledge Prof. Vinod Subramaniam and Prof. Herbert van Amerongen for providing me with the samples and giving me the permission to use their facilities. I would like to acknowledge Prof. Joost Meijers for generously supplying me with materials and allowing me to use their facilities in his lab, and Tom Plug who kindly helped me with performing the measurements. I appreciate our collaboration with Prof. Marcellus Ubbink, Prof. Claudio Toniolo, and Dr.
Dmitri Filoppov, from which I learned a lot. I would like to acknowledge the contribution of Dr. Ine Segers-Nolten, Nico Meeuwenoord, Ruud Spruijt, and Cor Wolfs in the preparation of the samples and for providing me with the initial measurements, which saved me a lot of time.
I am thankful to Henriette van Leeuwen, for her support in all the administrative matters, and to Jos Disselhorst and Harmen van der Meer for their technical assistance. I am grateful to Siva Kanaku at Expeditie, and Frans Folst and Tom Jansen at the Leidse Instrumentmakers School who prepared the delicate glass tubes for me and helped me with the last-important step in the preparation of the degassed samples. I thank Arno van Amersfoort and Ruud Kuyvenhoven for the maintenance of the computers and the computer network.
I would like to thank all my friends and colleagues at Leiden University, at Leiden
Observatory, in the Biophysics group, and especially in the MoNOS group for
turning the work environment into a sociable and friendly place. Diagonalization,
Hamiltonian, and many other expressions would have stayed strange terms for me,
if Dr. Silvia Sottini would not have spent her time on sharing her knowledge with
me. The simplified sketches and drawings of the instruments in my lab books are
the results of the fruitful discussions that I had with Dr. Huib Blok. I would like to
thank Martin van Son and Sandra Scanu, not only for their scientific contribution,
but also for being good and caring friends. I am also thankful to Faezeh Nami for
encouraging me to sport actively, which helped me a lot to reduce the work
pressure.
Aknowledgement
