The ORCA-ome as a key to understanding alkaloid biosynthesis in Catharanthus roseus
Hasnain, G.
Citation
Hasnain, G. (2010, June 29). The ORCA-ome as a key to understanding alkaloid biosynthesis in Catharanthus roseus. Retrieved from
https://hdl.handle.net/1887/15735
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/15735
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The ORCA-ome as a key to understanding alkaloid biosynthesis in Catharanthus roseus
Ghulam Hasnain
Ghulam Hasnain
The ORCA-ome as a key to understanding alkaloid biosynthesis in Catharanthus roseus ISBN 978-90-8570-582-6
Printed by: Wöhrmann Print Service
Cover designed and painted by Muhammad Khurshid
The ORCA-ome as a key to understanding alkaloid biosynthesis in Catharanthus roseus
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof. mr. P. F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op dinsdag 29 juni 2010 klokke 13:45 uur
door
Ghulam Hasnain geboren te Khaplu (Pakistan)
in 1974
Promotiecommissie
Promotores: Prof. dr. J. Memelink Prof. dr. R. Verpoorte Overige leden: Prof. dr. P.J.J. Hooykaas
Prof. dr. H.J. Bouwmeester (Wageningen University) Dr. H.J.M. Linthorst
Dr. A. Goossens (Ghent University, Belgium)
To my teacher Prof. Dr. Zahoor Ahmed Swati
Contents
Page
Chapter 1 9
Chapter 2 27
Chapter 3 49
Chapter 4 65
Chapter 5 81
Chapter 6 99
Summary 115
Samenvatting 121
Curriculum vitae 127
Biosynthesis of terpenoid indole alkaloids in Catharanthus roseus Genome-wide determination of ORCA target genes in Catharanthus roseus
ORCA2 and ORCA3 differentially regulate specific metabolic sub-pathways in the terpenoid indole alkaloid biosynthetic network Cloning and functional analysis of cathenamine reductase from Catharanthus roseus
Characterization of acyclic monoterpene primary alcohol : NADP+ oxidoreductases from Catharanthus roseus
Characterization of two ORCA-regulated genes, encoding enzymes belonging to the α/β hydrolase and carboxylesterase superfamilies, respectively
