Close the Gap : a study on the regulation of Connexin43 gap junctional communication
Zeijl, L. van
Citation
Zeijl, L. van. (2009, May 14). Close the Gap : a study on the regulation of Connexin43 gap junctional communication. Retrieved from
https://hdl.handle.net/1887/13799
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/13799
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A study on the regulation of Connexin43 gap junctional communication
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. P.F. van der Heijden,
volgens besluit van het College voor Promoties te verdedigen op donderdag 14 mei 2009
klokke 13.45 uur
door
Leonie van Zeijl
geboren te ‘s Hertogenbosch in 1975
Close the Gap
Promotiecommissie
Promotor Prof. Dr. W.H. Moolenaar
Leescommissie Prof. Dr. J.J. Neefjes Prof. Dr. P. ten Dijke Dr. K. Jalink
Het Nederlands Kanker Instituut
© Leonie van Zeijl, 2009
Cover: Co-staining of microtubules and Cx43 in migrating Rat-1 fibroblasts visualised by immunofluorescence
Cover design: Jessica van Essen and Leonie van Zeijl
The studies described in this thesis were performed at the Nether- lands Cancer Insitute (NKI/AvL), division of Cellular Biochemstry, Am- sterdam, the Netherlands.
Financial support was provided by the Dutch Cancer Society (KWF), grant nr. NKI 2000-2258 and the Centre for Biomedical Genetics (CBG)
Publication of this thesis was supported by the Dutch Cancer Society (KWF) and the Netherlands Cancer Insitute
Printed by: Wöhrmann Print Service, Zutphen
Contents
Chapter 1 General introduction 5
Chapter 2 Regulation of Connexin43 gap junctional communication by phosphatidylinositol 4,5 bisphosphate
35
Chapter 3 Inhibition of Connexin43 gap junctional communication by G(q)-coupled receptors: A critical role for residue Tyr265
63
Chapter 4 Dual regulation of Connexin43 gap junctional communication by GPCRs: A key role for ubiquitin ligase Nedd4
79
Chapter 5 Dependence of cell migration on Connexin43: modulation of N-cadherin expression
97
Summary 115
Samenvatting 121
List of abbreviations 129
Curriculum vitae 131
List of publications 133
Acknowledgements 135
