Dynamics and structural features of the microtubule plus- ends in interphase mouse fibroblasts
Zovko, S.
Citation
Zovko, S. (2010, June 22). Dynamics and structural features of the microtubule plus-ends in interphase mouse fibroblasts. Retrieved from https://hdl.handle.net/1887/15711
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
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Dynamics and structural features of the microtubule plus-ends in interphase mouse
fibroblasts
Sandra Zovko
Cover design: “Endless gallery” by Sanja Zovko
ISBN 978-90-8570-574-1
© 2010, Sandra Zovko. All rights reserved. No part of this thesis may be reproduced or transmitted in any form, by any means, electronic or mechanical, without prior written permission of the author.
Printed by Wöhrmann Print, Zutphen, The Netherlands
Dynamics and structural features of the microtubule plus-ends in interphase mouse
fibroblasts
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. P.F. van der Heijden,
volgens besluit van het College voor Promoties te verdedigen op dinsdag 22 juni 2010
klokke 13.45 uur
door
Sandra Zovko
Geboren te Tuzla, Bosnië en Herzegovina
in 1978
Promotiecommissie:
Promotor: Prof. Dr. Jan Pieter Abrahams LIC, Leiden
Co-promotor: Dr. Niels Galjart Erasmus University, Rotterdam Dr. Mieke A. Mommaas LUMC, Leiden
Overige leden: Prof. Dr. Marileen Dogterom Universiteit Leiden Prof. Dr. Bob van de Water LACDR, Leiden Prof. Dr. Mathieu Noteborn Universiteit Leiden
Dr.Thomas Surrey EMBL Heidelberg
Dr. Erno Vreugdenhil LACDR, Leiden
Printing of this thesis was financially supported by:
Jurriaanse Stichting
Stichting tot Bevordering van de Electronenmicroscopie in Nederland (SEN)
“What makes the desert beautiful is that somewhere it hides a well.”
Antoine de Saint-Exupery
Table of contents
Chapter I
General introduction and scope of the thesis 9
Chapter II
Electron Microscopy and Electron Tomography as a tool
to study the microtubules and microtubule plus-end conformations 33
Chapter III
Microtubule plus-end conformations
and dynamics in the periphery of interphase mouse fibroblasts 55
Chapter IV
Cryo-electron tomography of vitrified fibroblasts:
microtubule plus ends in-situ 79
Chapter V
Role of CLIP-170 and CLIP-115 in microtubule dynamics
and cellular morphology of interphase mouse fibroblasts 97
Chapter VI
General discussion and Summary 131
Nederlandse samenvatting 141
Abbreviations 147
List of publications 149
Curriculum vitae 151
Acknowledgments 153
