Human mesenchymal stromal cells : biological characterization and clinical application
Bernardo, M.E.
Citation
Bernardo, M. E. (2010, March 4). Human mesenchymal stromal cells : biological
characterization and clinical application. Retrieved from https://hdl.handle.net/1887/15034
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/15034
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Human Mesenchymal Stromal Cells: Biological Characterization and Clinical Application
2
Colofon
About the cover:
© Rene Magritte, Ceci n’est pas une pomme, c/o Pictoright Amsterdam 2010 Collection Scheringa Museum of Realism, The Netherlands
Printed by Wöhrmann Print Service, Zutphen, The Netherlands
The research presented in this thesis have been performed, in part, in Leiden at the Departments of Hematology (Prof. R. Willemze) and Immuno- Hematology and Blood Transfusion, LUMC (Prof. W.E. Fibbe) and, in part, in Pavia at the Department of Pediatric Onco-Hematology, Fondazione IRCCS Policlinico San Matteo (Prof. F. Locatelli). This work has been carried out in collaboration with the Departments of Pediatrics, Sub- Department Immunology, Haematology, Oncology, Bone Marrow Transplantation and Autoimmune diseases, LUMC, Leiden, The Netherlands and Division of Clinical Immunology and Centre for Allogeneic Stem Cell Transplantation, Karolinska Institute, Huddinge University, Stockholm, Sweden. The study has been supported by the School of Specialization in Pediatrics, University of Pavia (Prof. G. Rondini).
© M.E. Bernardo, Leiden, the Netherlands. All rights reserved. No part of this book may be reproduced or transmitted, in any form or by any means, without the written permission of the author.
ISBN: 978-90-8570-505-5
The printing of this thesis was financially supported by Interlabo Srl, Treviglio (BG), Italy.
Address for correspondence:
Maria Ester Bernardo, Onco-ematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo. V.le Golgi, 2. 27100 Pavia, Italy. Phone: +39-0382- 502607; fax: +39-0382-501251.
E-mail: mebernardo@gmail.com
Human Mesenchymal Stromal Cells:
Biological Characterization and Clinical Application
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. P.F. van der Heijden,
volgens besluit van het College voor Promoties te verdedigen op donderdag 4 maart 2010
klokke 10.00 uur.
door
Maria Ester Bernardo geboren te Voghera, Italië
in 1975
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Promotie commissie
Promotores: Prof. Dr. W.E. Fibbe
Prof. F. Locatelli (University of Pavia, Italy)
Overige Leden: Prof. Dr. R.M. Egeler Prof. Dr. A.J. Rabelink
Prof. F. Dazzi (Imperial College, London)
Ai miei genitori
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TABLE OF CONTENTS
CHAPTER 1: GENERAL INTRODUCTION 9
Published with minor modifications in Ann N Y Acad Sci. (2009;1176:101- 117. Review)
CHAPTER 2 45
Human mesenchymal stem cells derived from bone marrow display a better chondrogenic differentiation compared with other sources.
Connect Tissue Res. 2007;48:132-140.
CHAPTER 3 65
Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: further insights in the search for a fetal calf serum substitute.
J Cell Physiol. 2007; 211:121-130.
CHAPTER 4 97
Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms.
Cancer Res. 2007;67:9142-9149.
CHAPTER 5 123
Co-transplantation of ex-vivo expanded mesenchymal stem cells accelerates lymphocyte recovery and may reduce the risk of graft failure in haploidentical hematopoietic stem cell transplantation.
Blood. 2007;110:2764-2767.
CHAPTER 6 135
Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study.
Lancet. 2008;371:1579-1586.
CHAPTER 7 159
Phenotypical/functional characterization of in vitro expanded mesenchymal
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CHAPTER 8 185
Generation of mesenchymal stromal cells in the presence of platelet lysate:
a phenotypical and functional comparison between umbilical cord blood- and bone marrow-derived progenitors.
Haematologica. 2009;94:1649-1660. Epub 2009 Sep 22.
CHAPTER 9: GENERAL DISCUSSION 221
List of abbreviations 249
Summary in Dutch 251
Summary in English 253
Summary in Italian 255
Curriculum vitae 257
List of publications 258
