University of Groningen
Thermodynamic and stoichiometric constraint-based inference of metabolic phenotypes
Leupold, Karl Ernst Simeon
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Publication date: 2018
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Leupold, K. E. S. (2018). Thermodynamic and stoichiometric constraint-based inference of metabolic phenotypes. University of Groningen.
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Thermodynamic and stoichiometric
constraint-based inference of
metabolic phenotypes
Karl Ernst Simeon Leupold
2018The work published in this thesis was carried out in the research group Molecular Systems Biology of the Groningen Biomolecular Science and Biotechnology Institute (GBB) of the University of Groningen, The Netherlands. The research was financially supported by the BE-BASIC consortium.
ISBN: 978-94-034-1035-7 (printed version) ISBN: 978-94-034-1034-0 (electronic version)
Copyright © 2018 Karl Ernst Simeon Leupold
All rights reserved. No part of this publication may be produced, stored in a retrieval system of any nature, or transmitted in any form or by any means, elec-tronic, mechanical, including photocopying and recording, without prior written permission of the author.
Thermodynamic and stoichiometric
constraint-based inference of
metabolic phenotypes
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on Tuesday 18 September 2018 at 09.00 hours
by
Karl Ernst Simeon Leupold
born on 17 March 1987 in Berlin, Germany
Supervisors
Prof. M. Heinemann Prof. A.J.M. Driessen
Assessment Committee
Prof. B. Jayawardhana Prof. B.M. Bakker Prof. F. Bruggeman
Table of Contents
5
Table of Contents
Chapter 1 Introduction and aim of this thesis 7
Chapter 2 An upper limit in Gibbs energy dissipation governs cellular metabolism 19 Chapter 3 thermodynamic-based tool for flux predictions in Implementation and application of a new
genome-scale metabolic models 71 Chapter 4 Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan 97 Chapter 5 On the mechanistic reasons behind the observed limit in Gibbs energy dissipation of
cellular metabolism 129
Chapter 6 Conclusion and future perspective 143
Nederlandse samenvatting 146
