Neuropeptide receptor expression in inflammatory bowel disease
Beek, W.P. ter
Citation
Beek, W. P. ter. (2008, April 3). Neuropeptide receptor expression in inflammatory bowel disease. Retrieved from https://hdl.handle.net/1887/12667
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
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Neuropeptide Receptor Expression in
Inflammatory Bowel Disease
Pascale ter Beek
ISBN: 978-90-9022832-7
Printed by Gildeprint Drukkerijen B.V., Enschede, The Netherlands
© W.P. ter Beek, Leiden 2008
No part of this publication may be reproduced, stored in retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, and recording or otherwise, without prior written permission of the author.
The work described in this thesis was performed at the Department of Gastroenterology and
Hepatology of the Leiden University Medical Center (Leiden, The Netherlands). The project was partly financed by a grant from the Netherlands Digestive Diseases Foundation (WS 97-23).
Printing of this thesis was financially supported by the Section Experimental Gastroenterology (SEG) of the Netherlands Society of Gastroenterology (NVGE).
Neuropeptide Receptor Expression in
Inflammatory Bowel Disease
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. P.F. van der Heijden,
volgens besluit van het College voor Promoties te verdedigen op donderdag 3 april 2008
Klokke 16.15 uur
door
Willy Pascale ter Beek geboren te Almelo
in 1974
Promotiecommissie
Promotor: Prof. dr. C.B.H.W. Lamers
Co-promotor: Dr. ir. I. Biemond
Referent: Prof. dr. J.B.M.J. Jansen, Universitair Medisch Centrum St. Radboud, Nijmegen
Overige leden: Prof. dr. A.A.M. Masclee, Universiteit Maastricht
Prof. dr. G.J.A. Offerhaus, Universitair Medisch Centrum Utrecht Prof. dr. H. Morreau
Dr. ir. H.W. Verspaget
Contents
Abbreviations 7
Chapter one Introduction 9
Chapter two Outline and aims of this thesis 37
Chapter three Substance P receptor expression in patients with inflammatory bowel disease
41
Chapter four Quantification of neurotensin binding sites at different locations in inflammatory bowel disease and control human intestine
55
Chapter five Identification of neurotensin binding sites: Neurotensin receptor-1 and -3 are expressed in the human
gastrointestinal tract
69
Chapter six Gastrin-releasing peptide receptor expression is decreased in Crohn's disease but not in ulcerative colitis
85
Chapter seven Motilin receptor expression in smooth muscle, myenteric plexus and mucosa of human inflamed and noninflamed intestine
101
Chapter eight Summarizing discussion 119
Chapter nine Samenvatting 129
Nawoord 141
Curriculum Vitae 143
Abbreviations - 7
Abbreviations
5-HT Serotonin
Ach Acetylcholine
ATP Adenosine triphosphate
BN Bombesin
BRS Bombesin receptor subtype BSA Bovine serum albumin
CD Crohn’s disease
cDNA complementary desoxyribonucleic acid ENS Enteric nervous system
GALT Gut-associated lymphoid tissue GPR38-A G-protein coupled receptor 38-A GRP Gastrin-releasing peptide H2O2 Hydrogen peroxidase IBD Inflammatory bowel disease
IL Interleukin
Kd Dissociation constant
MPO Myeloperoxidase
mRNA Messenger ribonucleic acid
NK Neurokinin
NMB Neuromedin B
NRS Normal rat serum
NT(R) Neurotensin (receptor)
RT-PCR Reverse-transcriptase polymerase chain reaction SEM Standard error of the mean
SP Substance P
TBS Tris-buffered saline
TNBS Trinitrobenzenesulfonic acid UC Ulcerative colitis
