University of Groningen
Self-assembling nanofiber hydrogels to attenuate epithelial mesenchymal transition in lens
epithelial cells
da Cruz Barros, Raquel Sofia
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Publication date:
2018
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Citation for published version (APA):
da Cruz Barros, R. S. (2018). Self-assembling nanofiber hydrogels to attenuate epithelial mesenchymal
transition in lens epithelial cells. University of Groningen.
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Self-assembling nanofiber hydrogels
to attenuate epithelial mesenchymal
transition in lens epithelial cells
Self-assembling nanofiber hydrogels to attenuate epithelial mesenchymal transition in lens epithelial cells
By Raquel Sofia da Cruz Barros Groningen, 2018
ISBN
978-94-034-0485-1 (printed version)
978-94-034-0484-4 (digital version)
Copyright content
Copyright © 2018 by Raquel Sofia da Cruz Barros
All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means without the permission of the author and, when appropriate, the publisher holding the copyrights of the published articles.
Cover
Cover design by Loes Kema, GVO drukkers & vormgevers B.V. Printed by GVO drukkers & vormgevers B.V.
Self-assembling nanofiber hydrogels
to attenuate epithelial mesenchymal
transition in lens epithelial cells
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Wednesday 4 April 2018 at 12.45 hours
by
Raquel Sofia da Cruz Barros
born on 27 November 1985Self-assembling nanofiber hydrogels to attenuate epithelial mesenchymal transition in lens epithelial cells
By Raquel Sofia da Cruz Barros Groningen, 2018
ISBN
978-94-034-0485-1 (printed version)
978-94-034-0484-4 (digital version)
Copyright content
Copyright © 2018 by Raquel Sofia da Cruz Barros
All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means without the permission of the author and, when appropriate, the publisher holding the copyrights of the published articles.
Cover
Cover design by Loes Kema, GVO drukkers & vormgevers B.V. Printed by GVO drukkers & vormgevers B.V.
Self-assembling nanofiber hydrogels
to attenuate epithelial mesenchymal
transition in lens epithelial cells
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Wednesday 4 April 2018 at 12.45 hours
by
Raquel Sofia da Cruz Barros
born on 27 November 1985Supervisor
Prof. H.J. Busscher
Co-supervisor
Dr. T.G. van Kooten
Assessment Committee
Prof. R.A. Bank Prof. K.N. Faber Prof. O. Stachs
Paranimfen
Hilde Steg
Supervisor
Prof. H.J. Busscher
Co-supervisor
Dr. T.G. van Kooten
Assessment Committee
Prof. R.A. Bank Prof. K.N. Faber Prof. O. Stachs
Paranimfen
Hilde Steg
Para o meu pilar, papá, mamã e Sílvia To my cornerstone, daddy, mum and Sílvia
Para o meu pilar, papá, mamã e Sílvia To my cornerstone, daddy, mum and Sílvia
TABLE OF CONTENTS
Chapter 1 – Introduction
1.1. Epithelial Mesenchymal Transition and its regulators 12
1.2. Aims of the thesis 23
Chapter 2 - Self-assembled nanofiber coatings for controlling cell responses (Journal of Biomedical Materials Research Part A. 2017;105:2252-65) 31
Chapter 3 - Epithelial-mesenchymal transition in lens epithelial cells attenuated by exposure to functionalized self-assembling nanofiber gels (Submitted to Experimental Eye Research. 2017) 83
Chapter 4 - EMT reduction in an ex vivo lens model by using bioactive nanofiber-based hydrogels (Submitted to Journal of Cataract and Refractive Surgery. 2017) 111
Chapter 5 - Investigation of friction-induced damage to the pig cornea (The Ocular Surface. 2015; 13:315-20) 133
Chapter 6 - General discussion and conclusion 147
Summary 159
Samenvatting 163
Sumário 167
Acknowledgements 171
TABLE OF CONTENTS
Chapter 1 – Introduction
1.1. Epithelial Mesenchymal Transition and its regulators 12
1.2. Aims of the thesis 23
Chapter 2 - Self-assembled nanofiber coatings for controlling cell responses (Journal of Biomedical Materials Research Part A. 2017;105:2252-65) 31
Chapter 3 - Epithelial-mesenchymal transition in lens epithelial cells attenuated by exposure to functionalized self-assembling nanofiber gels (Submitted to Experimental Eye Research. 2017) 83
Chapter 4 - EMT reduction in an ex vivo lens model by using bioactive nanofiber-based hydrogels (Submitted to Journal of Cataract and Refractive Surgery. 2017) 111
Chapter 5 - Investigation of friction-induced damage to the pig cornea (The Ocular Surface. 2015; 13:315-20) 133
Chapter 6 - General discussion and conclusion 147
Summary 159
Samenvatting 163
Sumário 167
Acknowledgements 171
