A metabolomic approach to thrips resistance in tomato Romero González, R.R.

A metabolomic approach to thrips resistance in tomato

Romero González, R.R.

Citation

Romero González, R. R. (2011, October 11). A metabolomic approach to thrips resistance in tomato. Retrieved from https://hdl.handle.net/1887/17920

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A metabolomics approach to thrips resistance in tomato

by Roman R. Romero González

A metabolomics approach to thrips resistance in tomato

PROEFSCHRIFT

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. P. F. van der Heijden,

volgens besluit van het College voor Promoties te verdedigen op dinsdag 11 oktober 2011

klokke 13:45 uur

door

Roman Rodolfo Romero González

geboren te Maracaibo (Venezuela) in 1974

P r o m o t i e c o m m i s s i e

Promotors • Prof. dr. R. Verpoorte Prof. dr. P. G. L. Klinkhamer

Co-promotors • Dr. Y. H. Choi Dr. K. A. Leiss

Overige leden • Prof. dr. N. M. van Dam (Universiteit Nijmegen) Prof. dr. T. A. van Beek (Universiteit Wageningen) Prof. dr. C. J. ten Cate

Dr. H. K. Kim

This work was financially supported by Universidad de Los Andes, Mérida, Venezuela.

Cover design and thesis lay-out by Rene Glas (www.reneglas.com) and Roman Romero.

Main picture: young leaf of Solanum lycopersicum with adult and larval thrips. Framed pictures:

main types of glandular trichomes in Solanum.

Printed by Wörhmann Print Service, Zutphen ISBN: 978-90-8570-779-0

C o n t e n t s

Chapter 1 •

General introduction 7

Chapter 2 •

Metabolomic analysis of host-plant resistance to thrips in wild and domesticated tomatoes

13

Chapter 3 •

Tomato’s best thrips shield: acylsugars, sesquiterpenes, methylketones or phenolics?

25

Chapter 4 •

Thrips resistance and NMR-based metabolic profiling of a Solanum pennellii x lycopersicum introgression population

43

Chapter 5 •

Salting-out gradients in centrifugal partition chromatography for the isolation of chlorogenic acids from green coffee beans

59

Chapter 6 •

Effect of chlorogenic acid on thrips performance in artificial diet bioassays

73

References 83

Summary and general discussion 97

Samenvatting 101

Resumen 105

Curriculum vitae 109

List of publications 111

A mi amada familia