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(1)

B.

32.7%

CD94

NKG2A

CD4

+

T cells

CD8

+

T cells

NK cells

0.087% 76.5%

A.

0 2 0 4 0 6 0 8 0 1 00 PBMC TIL * * * * PBMC TIL % NKG2A + 0 .0 0 .5 1 .0 2 0 4 0 6 0 8 0 1 00 * * 0 2 0 4 0 6 0 8 0 1 00 PBMC TIL * * *

D.

Overall survival

(2)

D.

NKG2A

CD8α

CD8β

CD56

NKp44

NKp46

CD16

CD161

CD45RA CD45RO KLRG1

CCR7

CD127

CD27

CD28

CD103 HLA-DR

CD38

CD25

PD-1

CD7

CD122

tSNE1 tSNE2 high low N K G 2A C D 5 6 C D 2 5 N K p 4 4 C D 10 3 C D 1 2 2 C D 7 H L A -D R C D 1 6 1 T C R γ δ c -K IT C D 1 6 P D -1 C D 8 β C D 3 8 C D 8 α C D 1 2 7 C R T H 2 C D 3 N K p 4 6 C C R 6 C D 4 5 R O C D 4 5 C D 4 5 R A C D 2 8 C D 2 7 C C R 7 K LR G 1 -1 0 1

Expression ratio of NKG2A

(3)

A.

0 8 24 29 36

TC-1 tumor intratumoural analysis

peptide vaccination days: NKG2A relative counts

B.

72.3 % 73.7 %

NK cells

0.41 % 2.02 %

CD4 T cells

13.7 % 41.4 %

CD8 T cells

% NKG2A + 0 2 0 4 0 6 0 8 0 1 00 * * ** * * *

C.

untreated (d24)vaccinated (d24)vaccinated (d29)vaccinated (d36)untreated (d24)vaccinated (d24)vaccinated (d29)vaccinated (d36) untreated (d24)vaccinated (d24)vaccinated (d29)vaccinated (d36)

D.

0 2 0 4 0 6 0

0

1000 untreated

vaccinated

Days after tumor challenge

(4)

C.

F.

Untreated Vaccinated + isotype Vaccinated + α-NKG2A α-NKG2A % survival

Days after tumor challenge

** 0 2 0 4 0 6 0 0 5 0 1 00 % survival

Days after tumor challenge

(5)

A.

B16

TC-1

0 2 0 4 0 6 0 8 0 0 5 0 1 00 ** % survival Untreated Vaccinated + isotype Vaccinated + α-NKG2A

TC-1, Qa-1

b-/-

host

Days after tumor challenge

(6)

RMA

D.

0 2 0 4 0 6 0 0 5 0 1 00 untreated vaccinated untreated

Qa-1

b -vaccinated * % survival

Days after tumor challenge

WT

0 2 0 4 0 6 0 0 5 0 1 00 untreated adjuvant alone vaccinated untreated adjuvant alone vaccinated

Qa-1

b

-Days after tumor challenge

% survival

B16

*

WT

F.

TC-1

0 2 0 4 0 6 0 0 1 ,00 0 untreated vaccinated

Qa-1

b

-Days after tumor challenge

A.

0 1 ,00 0

WT

tumor size (mm 3)

Days after tumor challenge

0 2 0 4 0 6 0 0/16 0/16 0/260/17 0 1 ,00 0 WT Qa-1 b -WT Qa-1 b -tumor size (mm 3) *

Day 18

0 1 00 2 00 3 00 WT Qa-1 b -* -*

Day 28

untreated vaccinated

B.

C.

0 2 0 4 0 6 0 0 200 tumor size (mm 3)

WT

3/20 4/20

Days after tumor challenge 0 2 0 4 0 6 0

0 200

untreated vaccinated

Days after tumor challenge

Qa-1

b -4/20 8/20 0 2 0 4 0 6 0 0 2 00 untreated adjuvant alone vaccinated 0/8 0/6 3/6

Qa-1

b -tumor size (mm 3)

Days after tumor challenge

E.

0 2 0 4 0 6 0 0 200 0/8 0/8 0/7

WT

tumor size (mm 3)

(7)

0 0 1 00

B.

0 2 0 4 0 6 0 0 1 00 vaccinated vaccinated, NK-depleted control vaccinated

Qa-1

b -% survival

Days after tumor challenge

ns 0 2 0 4 0 6 0 0 200 vaccinated vaccinated NK depleted control vaccinated

WT

0/10 0/10 4/20 tumor size (mm 3) 0 2 0 4 0 6 0 0 200 vaccinated vaccinated NK depleted control vaccinated

Qa-1

b -1/10 3/10 8/20 tumor size (mm 3) 2 0 4 0 6 0 vaccinated vaccinated NK depleted control vaccinated

WT

% survival ns

A.

C.

0 5 1 0 1 5 % IFN-γ / CD8

WT

ns peptide - + - + - + vaccinated vaccinated

NK depletedvaccinatedcontrol

0 5 1 0 1 5 peptide - + - + - + % IFN-γ / CD8 vaccinated vaccinated

NK depletedvaccinatedcontrol

Qa-1

b

-ns

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