Mesoporous silica nanoparticles with large pores for the encapsulation and release of proteins

(1)

Mesoporous silica nanoparticles with large pores for the

encapsulation and release of proteins

Citation for published version (APA):

Tu, J., Boyle, A. L., Friedrich, H., Bomans, P. H. H., Bussmann, J., Sommerdijk, N. A. J. M., Jiskoot, W., & Kros, A. (2016). Mesoporous silica nanoparticles with large pores for the encapsulation and release of proteins. ACS Applied Materials & Interfaces, 8(47), 32211-32219. https://doi.org/10.1021/acsami.6b11324

DOI:

10.1021/acsami.6b11324

Document status and date: Published: 30/11/2016 Document Version:

Accepted manuscript including changes made at the peer-review stage Please check the document version of this publication:

• A submitted manuscript is the version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.

• The final author version and the galley proof are versions of the publication after peer review.

• The final published version features the final layout of the paper including the volume, issue and page numbers.

Link to publication

General rights

Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain

• You may freely distribute the URL identifying the publication in the public portal.

If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license above, please follow below link for the End User Agreement:

www.tue.nl/taverne

Take down policy

If you believe that this document breaches copyright please contact us at:

openaccess@tue.nl

providing details and we will investigate your claim.

(2)

S-1

Supporting Information

Mesoporous Silica Nanoparticles with Large Poresfor the Encapsulation and Release of Proteins

Jing Tu a, Aimee L. Boyle a, Heiner Friedrich b, Paul H. H. Bomans b, Jeroen Bussmann a, c,

Nico A. J. M. Sommerdijk b, Wim Jiskoot d*, Alexander Kros a*

a_{Department of Supramolecular & Biomaterials Chemistry, Leiden Institute of Chemistry}

(LIC), Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands

b_{Laboratory of Materials and Interface Chemistry & Center of Multiscale Electron}

Microscopy, Department of Chemical engineering and Chemistry, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB

Eindhoven, The Netherlands

c_{Leiden Institute of Biology, Gorlaeus Laboratories, Leiden University, P.O. Box 9502,}

Leiden, 2300 RA, The Netherlands

d_{Division of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre}

for Drug Research (LACDR), Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands

*_{Corresponding Authors}_{a.kros@chem.leidenuniv.nl}

w.jiskoot@lacdr.leidenuniv.nl

(3)

S-2

Figure S1. LAC, OVA, BSA and CAT release profiles from AEP-MSNs in (a) 1 mM PB (ionic strength 12 mM), and (b) 1 mM PB with 0.9% NaCl at 37 °C (ionic strength 166 mM); Hb, LYS and CYT release profiles from MSNs in (c) 1 mM PB (ionic strength 12 mM), and (d) 1 mM PB with 0.9% NaCl at 37 °C (ionic strength 166 mM).

(4)

(5)

S-4

Figure S2. CD spectra of free proteins (black) and the released proteins from MSNs (or AEP-MSNs) (red) in PBS, pH 7.4, 25 °C. Concentration of non-encapsulated and encapsulated proteins were matched to provide accurate comparisons.

Table S1. Physical characteristics of MSNs and AEP-MSNs

Sample

BET surface area

(m2/g) Channel volume (cm3/g) Channel diameter (nm)a MSNs 506 1.01 10 ± 1 MSNs/CYT 263 0.56 7.5 ± 1.5 MSNs/Hb 275 0.58 7.5 ± 1.5 AEP-MSNs 318 0.71 9 ± 1 AEP-MSNs/LAC 203 0.42 6.5 ± 1.5 AEP-MSNs/CAT 223 0.46 6.5 ± 1.5

a_{Calculated from the desorption branch of the N2 sorption isotherms based on the BJH method.}