University of Groningen
Engineered Proline-based Carboligases With Improved Catalytic Properties
Guo, Chao
DOI:
10.33612/diss.160297693
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Publication date: 2021
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Guo, C. (2021). Engineered Proline-based Carboligases With Improved Catalytic Properties. University of Groningen. https://doi.org/10.33612/diss.160297693
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PROPOSITIONS
Belonging to the thesis
Engineered Proline-based Carboligases With Improved
Catalytic Properties
by Chao Guo
1. Enzyme promiscuity is an important starting point for the engineering of novel biocatalysts for chemical synthesis. (this thesis)
2. Systematic mutagenesis of the entire enzyme is a powerful strategy to identify functional ‘hotspot’ positions that can be used as targets for further combinatorial mutagenesis to yield superior enzymes with improved catalytic properties. (this thesis)
3. The members of the 4-OT family of enzymes can function as novel carboligases, promoting synthetically useful asymmetric aldol and Michael-type addition reactions. (this thesis)
4. 4-OT and its family members may promote various distinct bond-forming reactions via reactive iminium ion intermediates. (my view)
5. Don't compare yourself to others, but try to be a better you. (my view) 6. The journey of pursuing a PhD is not only teaching me how to answer
scientific questions, but also teaching me the way of addressing problems. (my view)
7. Dedication is the most important personality for being a true scientist. (my view)
